Journal: International Journal of Molecular Sciences
Article Title: Wrinkle-Improving Effect of Novel Peptide That Binds to Nicotinic Acetylcholine Receptor
doi: 10.3390/ijms25147860
Figure Lengend Snippet: Stimulation of collagen production in cultured dermal fibroblasts, and expressions of AQP3 and HAS2 in keratinocyte in vitro. ( a ) Increased production of collagen by our peptide was observed in cultured human dermal fibroblasts by enzyme-linked immunosorbent assay (ELISA). mRNA expression of ( b ) AQP3 ( c ) and HAS2 in keratinocytes treated with our peptide (*: p < 0.05; **: p < 0.01).
Article Snippet: The Human Pro-Collagen α1 DuoSet ® ELISA kit used in this test was from R&D Systems (Minneapolis, MN, USA) and was used according to the experimental protocol provided by the manufacturer.
Techniques: Cell Culture, In Vitro, Enzyme-linked Immunosorbent Assay, Expressing